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Antibodies sure to the blocking peptide not bind to the epitope about the target protein. By comparing the staining in the blocked antibody versus the antibody on your own, one can see which staining is specific.
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This information outlines further attainable optimization of mobile and immunoassay parameters to make sure the very best results are...
This information outlines further achievable optimization of mobile and immunoassay parameters to ensure the absolute best effects are...
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by the two antibodies...
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Blocking peptides are peptides that bind specially towards the concentrate on antibody and block antibody binding. These peptide typically is made up of the epitope regarded from the antibody.
Focus on Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E sorts a fancy with and features as being a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S transition. This protein accumulates for the G1-S phase boundary and is also degraded as cells progress via S period.
Overexpression of this gene has long been observed in several tumors, which ends up in chromosome instability, and therefore may perhaps contribute to tumorigenesis. This protein was identified to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a important function in endorsing cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have been described. Two more splice variants had been documented but detailed nucleotide sequence details will not be yet offered.
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